Home Health Letter for the article Detection of Antibiotic Resistance Genes in Pse

Letter for the article Detection of Antibiotic Resistance Genes in Pse

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We thank the authors for reporting their findings in Detecting Antibiotic Resistance Genes. Pseudomonas aeruginosa By whole genome sequencing published in Infection and Drug Resistance 2022:15 6703–6709”. This is very important information for whole genome sequencing using conventional PCR methods and susceptibility testing of lambs to antibiotic resistance. The purpose of the study itself was to evaluate resistance genes. Pseudomonas aeruginosa Results for the resistance genes are sul1, aac(3)-Ic, blaPAO, blaGES-1, blaGES-5 aph(3′)-XV, blaOXA-50, aacA4, catB7, aph(3′)-IIb, aadA6, fosA, tet(G), cmlA1, aac(6′)Ib-cr, and rmtF.1

The study also reported that: Pseudomonas aeruginosa It is resistant to some antibiotics and can be a threat in societies that use antibiotics irrationally. Pseudomonas aeruginosa Extended lactamase, carbapenemase, aminoglycoside converting enzyme, etc. The study also investigated the prevalence of her ARG in three individuals. Pseudomonas aeruginosa Strains using whole-genome sequencing indicated the PCR technique used Pseudomonas aeruginosa Resistance to various antibiotics such as ceftazidime, cefotaxime, cefepime, piperacillin/tazobactam, imipenem.2

Unfortunately, this study was unable to report the resistant genomic sequences in detail. Pseudomonas aeruginosaTwelve different dilutions of each antibiotic had to be tested by a two-fold dilution method (concentrations tested ranged from 1024 μg/mL to 0.5 μg/mL). For example, nalidixic acid (NAL), which represents the first generation. Ciprofloxacin (CIP), norfloxacin (NOR), and ofloxacin (OFL) represent the second generation.Using the ATCC27853 code for Levofloxacin (LEV) representing the 3rd generation, Gemifloxacin (GEM) and Moxifloxacin (MOX) representing the 4th generation (all from Sigma-Aldrich, USA) Pseudomonas aeruginosa Test their quality.3

There are also methods that can be used as comparisons for susceptibility testing. Pseudomonas Becton Dickinson (BD) Phoenix automated systems can be used to identify to species level (ID) and test for antimicrobial susceptibility (AST). This method uses a modified carbapenem inactivation method (mCIM) to analyze isolates produced by carbapenemase enzymes.The results of this study showed that 110 people Pseudomonas Biobank isolates were reanalyzed, 100 of which Pseudomonas and in it Pseudomonas aeruginosa The resistance rate was 98% and P. putida 2%. The highest rate of resistance was observed with ceftazidime (35%) and the lowest rate with amikacin (2%). Twenty-seven isolates were identified as test candidates for carbapenemase enzyme production, and only 3/27 (11%) isolates were detected as carbapenemase enzyme producers.Four

Disclosure

The authors report no conflicts of interest in this communication.

References

1. Ahmed OB. Detection of antibiotic resistance genes in Pseudomonas aeruginosa by whole-genome sequencing. Infect drug resist2022;15:6703–6709. doi:10.2147/IDR.S389959

2. Qin J, Zou C, Tao J, et al. Carbapenem-resistant Pseudomonas aeruginosa infection in elderly patients: antibiotic resistance profile, risk factors, and impact on clinical outcome. Infect drug resist2022;15:2301–2314. doi:10.2147/IDR.S358778

3. Molinaro M, Morelli P, De Gregori M, et al. Efficacy of intraventricular amikacin treatment in pan-resistant Pseudomonas aeruginosa postoperative meningitis. Infect drug resist2018;11:1369–1372. doi:10.2147/IDR.S169271

Four. Addis T, Araya S, Desta K. Multiple, widespread, outbreaks of pan-drug-resistant Pseudomonas aeruginosa and carbapenemase production from putative isolates deposited in the Biobank of the Ethiopian Institute of Public Health. Infect drug resist2021;14:3609–3618. doi:10.2147/IDR.S327652

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